Sunday, January 9, 2011
Definition of Chromatography
Chromatograhy may be defined as a methos of separating a mixture of components into individual components through equilibrium distribution between two phases.
Essentially, the technique of chromatography is based on the differences in the rate at which the components of a mixture move through a porous medium (called stationary phase) under the influence of some solvent or gas (called moving phase).
The chromatographic method of separation, in general , involves the following steps:
1. Adsorption or retention of a substance or substances on the stationary phase.
2. Separation of the adsorbed substances by the mobile phase.
3. Recovery of the separated substances by a continuous flow of the mobile phase, the method being called elution.
4. Qualitative and quantitative analysis of the eluted substances.
Essentially, the technique of chromatography is based on the differences in the rate at which the components of a mixture move through a porous medium (called stationary phase) under the influence of some solvent or gas (called moving phase).
The chromatographic method of separation, in general , involves the following steps:
1. Adsorption or retention of a substance or substances on the stationary phase.
2. Separation of the adsorbed substances by the mobile phase.
3. Recovery of the separated substances by a continuous flow of the mobile phase, the method being called elution.
4. Qualitative and quantitative analysis of the eluted substances.
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